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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has become observed in many tumors, which leads to chromosome instability, and therefore could lead to tumorigenesis. This protein was identified to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a crucial role in advertising and marketing cell-cycle progression while in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, are already explained. Two more splice variants were noted but in depth nucleotide sequence info just isn't but offered.
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Blocking peptides are peptides that bind exclusively to the target antibody and block antibody binding. These peptide typically incorporates the epitope regarded with the antibody.
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This guideline outlines additional possible optimization of mobile and immunoassay parameters to be sure the best possible results are...
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This guidebook outlines more attainable optimization of cellular and immunoassay parameters to be sure the absolute best benefits are...
Antibodies certain to the blocking peptide not bind for the epitope within the goal protein. By comparing the staining from the blocked antibody vs . the antibody by itself, you can see which staining is distinct.
Goal Info Cyclin E belongs towards the highly conserved cyclin spouse and children, whose users are characterised by a remarkable periodicity in protein abundance through the mobile cycle. Cyclins purpose as regulators of CDK kinases. Distinct cyclins exhibit distinctive expression and degradation designs which contribute towards the temporal coordination of every mitotic event. Cyclin E varieties a fancy with and functions for a regulatory subunit of CDK2, whose action is necessary for cell cycle G1/S changeover. This protein accumulates for the G1-S stage boundary and is degraded as cells development via S section.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by each antibodies...
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